as the other guide states this last mission is virtually impossible to do with out the glitch. the best i done was got it down to 20 seconds but, this is far better than getting frustrated and raging,
ok when you first put the antidote into the device, just simply use spider sense. hold the left stick down to the left and web zip.
the trick is to not see any of the lizards, that way they do not spawn.
i have heard that you can actually defeat them with black suited spiderman but you will need to do the whole game again which is pointless.
just follow this guide and the achievement should pop up.
(sorry about the poor qaulity but this is not my video)