Click the game.
Pause the recording when there's 0:49 left.
Click Start Playing.
As soon as you start playing, press
Select 'Quit Game'.
Select 'Quit (Save)'.
Use two electric bombs on the boss (it should be A button), and it should kill the boss. You may have to press B to select the electric bomb weapon.
This achievement will then pop. as you progress up the screen after the game says 'Area 13'.