To get this achievement, you need to defeat the "Supreme Hunter" boss, this battle comes at the end of missions 22 where you escort a tank to 3 buildings, kill any infected in the area trying to hurt it, and then let it blow up the hives.
Killing the supreme hunter is not a simple task, even on normal difficulty. The method I used was simple...
1) Jump around with the whipfist upgrade and use the "Y" attack (Which is like an extension attack) to hurt the supreme hunter. A minute of this, and the hunter will summon more enemies, along with some tanks busting through the walls.
2) Get to a tank, and try to hijack it (B). If successful (Sometimes the supreme hunter knocks you off), lock onto the supreme hunter (LB), and start shooting every missle (RB) you have at it..When you run out, get to another tank and repeat..
3) If you start running low on health, avoid the supreme hunter and consume the little infected, as the regular hunters take too many hits to weaken, and by the time you get them down to consumption point, the supreme hunter will be over beating the snot out of you.
4) I'm going to make an assumption and say it takes about 83 missles to kill the supreme hunter, I did not have to use more than 4 tanks (They start with 25 missles each) to take out the supreme hunter.
After he dies, you will recieve this achievement.