- Splash!
Destroy the Naval Cannon.
01 April 2011 - 3 guides
- Boom!
Destroy the Bomber.
01 April 2011 - 2 guides
- Kaboom!
Destroy the Tank.
01 April 2011 - 5 guides
- Serial Killer
Perform an Execution Streak of 10 kills.
Unlocked offline - 6 guides
- Bloody Sunday
Complete DNA Sequence 7.
Unlocked offline - 1 guide
- Vittoria Agli Assassini
Complete DNA Sequence 8.
Unlocked offline - 1 guide
- Requiescat In Pace
Complete DNA Sequence 9.
Unlocked offline - 1 guide
- A Knife to the Heart
Secure the Apple of Eden.
Unlocked offline - 2 guides
- Tower Offense
Burn All Borgia Towers.
Unlocked offline - 2 guides
- Show Off
Complete 10 Guild Challenges.
Unlocked offline - 3 guides
- Perfectionist
Earn 3 gold medals in the Animus Virtual Training Program.
Unlocked offline - 10 guides
- In Memoriam
Collect All Feathers.
Unlocked offline - 6 guides
- The Gloves Come Off
Win the highest bet at the Fights.
29 March 2011 - 6 guides
- Perfect Recall
Achieve 100% Synchronization in any Sequence other than Sequence 1.
29 March 2011 - 6 guides
- Forget Paris
Complete DNA Sequence 6.
26 March 2011 - 1 guide
- Fundraiser
Complete DNA Sequence 5.
25 March 2011 - 1 guide
- Principessa in Another Castello
Complete DNA Sequence 4.
25 March 2011 - 1 guide
- Spring Cleaning
Kill a guard with a broom.
25 March 2011 - 11 guides
- Home Improvement
Renovate 5 buildings in the Antico district.
23 March 2011 - 1 guide
- Bang!
Destroy the Machine Gun.
23 March 2011 - 1 guide
- Fixer-Upper
Complete DNA Sequence 3.
Unlocked offline - 2 guides
- Brotherhood
Recruit 3 Assassins.
Unlocked offline - 1 guide
- Your Wish is Granted
Throw money in a well.
Unlocked offline - 2 guides
- Job skills
During Open Conflict, kill your target and escape (Multiplayer Only).
16 December 2010 - 5 guides
- Rome in Ruins
Complete DNA Sequence 2.
10 December 2010 - 2 guides
- Golden Boy
Discover the Shrine in the Terme di Traiano.
10 December 2010 - 4 guides
- Déjà Vu
Replay a Memory.
09 December 2010 - 1 guide
- Mailer Daemon
Access your email in 2012.
09 December 2010 - 4 guides
- Dust to Dust
Find 1 Artifact in 2012.
09 December 2010 - 6 guides
- Sanctuary! Sanctuary!
Find a secure place to hide and re-enter the Animus.
09 December 2010 - 2 guides
- Battle Wounds
Complete DNA Sequence 1.
09 December 2010 - 2 guides
- Technical Difficulties
Attempt to access DNA Sequence 9 for the first time.
09 December 2010 - 4 guides
- Strong Closer
Take the lead 10 seconds before the end of a session and go on to win (Multiplayer Only).
04 December 2010 - 7 guides
- Fast Learner
Kill your target and escape your pursuer in less than 10 seconds (Multiplayer Only).
27 November 2010 - 4 guides
- Overachiever
Score 750 points or more on a single kill (Multiplayer Only).
27 November 2010 - 5 guides
- Needle in a Haystack
Kill your target while hidden in a hay bale (Multiplayer Only).
24 November 2010 - 2 guides
- Ahead of the Curve
Perform a double or triple escape (Multiplayer Only).
23 November 2010 - 3 guides
- Synchronization Established
Complete an entire Session with at least one kill (Multiplayer Only).
23 November 2010 - 1 guide
- .. .- -- .- .-.. .. ...- .
Solve all of Subject 16’s puzzles.
Locked - 5 guides
- Capture the Flag
Remove All Borgia Flags in Rome.
Locked - 3 guides