Here is the full list of all 27 Duke Nukem 3D: 20th Anniversary Edition World Tour achievements worth 1,000 gamerscore.
Complete "The Birth" on any difficulty.
Complete "Shrapnel City" on any difficulty.
Complete "Lunar Apocalypse" on any difficulty.
Find 70 secret areas.
Find Luke in the Level "Lunar Reactor"
Complete "L.A. Meltdown" on any difficulty.
Finish a secret level.
Find the Doomguy in the Level "Death Row"
Finish the "Hollywood Holocaust" level in under 3 minutes.
Carry all weapons with ammo at once.
Use a cheat code.
Finish any level on "Damn, I'm Good" difficulty.
Complete "Alien World Order" on any difficulty.
Step on 40 enemies after using the Shrinker on them.
Kill 1000 enemies.
Burn 50 bad guys to death.
Kill 5 bad guys with one shot.
Find Sam in "Mirage Barrage".
Kill 10 bad guys in 10 seconds while on steroids.
Listen to Developer Commentary (Use at least one trigger).
Use the Incinerator
Step in a pile of excrement.
Find a secret area.
Kill every enemy on a level.
Kill a Firefly Trooper while it's flying small.
Use the hookah in High Times.
Tip an exotic dancer.