- Recipe for Disaster
Complete Mission 2: "Light's Out" without activating any turrets.
- Cold Slither
Complete Mission 4: "Cold Case", without receiving health damage from Zartan.
- A Joe Needs No Tricks
Complete Mission 5: "Scarcity of Freedom" using the self-destruct sequence only once.
- A McCullen Won't Forget
During Mission 6: "Come With Me If You Want To Live", knockdown Destro at least 4 times.
- Man on the Run
Complete Mission 8: "It's a Trap!" with 2 minutes or more left.
- Close Enough to the End
Complete Mission 8: "It's a Trap!" without losing everything.
- That Tech is Expensive!
Complete Mission 8: "It's a Trap!" destroying no more than 3 B.A.T.S. pods.
- Survival of the Fittest
During Mission 9: "Made in Japan" as Storm Shadow, defeat most of the ninjas using melee attacks.
- Graves of the Firefly
During Mission 9: "Made In Japan" as Firefly, defeat most of the enemies using only the Boomslang.
- A Relic of the Past
During Mission 10: "Fury & Silence", don't lose your grasp on the mold.
On Mission 10: "Fury & Silence", deal the final blow to Storm Shadow using Snake Eyes' katana.
- You can't do thiss to me!
During Mission 11: "Nothing Personal", defeat Cobra Commander in less than 2 minutes.
- Dissasembly Line
During Mission 12: "First Light" defeat 25 B.A.T.'s using turrets.
- The Pride of the McCullen
Complete Mission 13: "Unplugged" as Destro without taking down neither Scarlett nor Lady Jaye.
- As Deadly as Ever
During Mission 13: "Unplugged" defeat both Lady Jaye and Scarlett while playing as Baroness.
- Spring Cleaning
Complete Mission 14: "Undo What They Did to USS" having defeated most enemies.
- They won't even know what hit them.
Complete Mission 16: "Enemy of my Enemy" in under 15 minutes.
- Target Practice
During Mission 16: "Enemy of my Enemy" on single player, win the B.A.T. destruction contest.
- Doesn't Ring a Bell
Complete Mission 17: "Freedom Squad" without losing control of your character.
- Snake is sneak spelled sideways.
During Mission 17: "Freedom Squad", defeat the last boss avoiding its special attack.
- Whenever There's Trouble
Complete all missions of story mode on "Action Figures" dificulty.
- G.I. Joe is There
Complete all missions of story mode on "Government Issue" difficulty.
- American Hero
Complete all missions of story mode on "Man of Action" difficulty.
- A Real American Hero
Complete all missions of story mode on "Real American Hero" difficulty.