Full list of all 46 The Amazing Spider-Man achievements worth 1,000 gamerscore. It takes around 15-20 hours to unlock all of the achievements on Xbox 360.
Collected all 700 Spider-Man Comic Pages
Defeated the S-01 without touching the ground
Completed all XTreme Race challenges
Completed all XTreme Video challenges
Cleared all car chases
Rescued all infected civilians
Acquired all upgrades
Completed all Oscorp Secret Research Labs
Defeated 1000 enemies
Returned all escapees to the police
Completed the game on human difficulty
Completed the game on hero difficulty
Completed the game on super hero difficulty
Defeated Lizard
Defeated Nattie
Rescued Alistaire Smythe
Defeated the S-03
Immobilized 6 enemies simultaneously with web
Resolved all police deadlocks
Cleared the second fight against the Hunter robots
Defeated Felicia
Defeated Scorpion in the city
Defeated Rhino in the sewers
Defeated 500 enemies
Performed a Web-Rush punch
Defeated 100 enemies by performing Stealth Takedowns
Defeated the S-02
Defeated Scorpion in quarantine
Defeated Iguana
Destroyed a Seeker before it could call a Hunter
Cleared the first fight against the Hunter robots
Defeated a Hunter without using your Web-Shooters
Defeated Vermin
Defeated 50 enemies by performing Stealth Takedowns
Achieved a combo streak of 42
Chained 10 Web-Rushes in the city
Defeated 100 enemies
Performed 25 Signature Moves
Saved a hostage caught in a petty crime
Defeated Rhino in the city
Defeated the S-01
Collect all magazines
Collect all hidden Tech Pieces
Collect all Oscorp Manuals
Collect all audio evidence
Unlock all concept art