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Reunited with your original Little Sister.

2 guidesOffline Game Mode - These achievements require play in game modes that do not necessitate a connection to any online services.Single Player - These achievements can be obtained by a single player.Main Storyline - These achievements are gained automatically by progressing through the main game modes.
Leo AscendentLeo Ascendent490,096
18 Feb 2010
21 1 0

You will get this at the end of the game.

Once you have defeated both Big Sisters and Gathered the Big Sis suit for Eleanor you will be returned to your body and she will come rescue you. Upon that, this will unlock, you will also get a Plasmid that allows you to summon her (as a Big Sis) so she can help you in fights.

And it's story based, and therefore, cannot be missed.